Effects of 8-bromo-7-methoxychrysin and sorafenib on apoptosis of liver cancer stem-like cells
10.3969/j.issn.2095-4344.2015.45.009
- VernacularTitle:8-溴-7-甲氧基白杨素和索拉非尼对肝癌干细胞样细胞凋亡的影响
- Author:
Haizhen ZHAO
;
Ruijun LIU
- Publication Type:Journal Article
- Keywords:
Carcinoma,Hepatocelular;
Apoptosis;
Cel Line,Tumor;
NF-kappa B;
Tissue Engineering
- From:
Chinese Journal of Tissue Engineering Research
2015;(45):7263-7267
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:We tried to combine 8-bromo-7-methoxychrysin and sorafenib in order to offset the tolerance of hepatocelular cancer stem cels to sorafenib, thereby comprehensively improving the therapeutic efficacy on hepatocelular carcinoma. OBJECTIVE:To observe the effects of 8-bromo-7-methoxychrysin, sorafenib and their combination on apoptosis of liver cancer stem-like cels SMMC-7721, and to analyze their mechanisms. METHODS:SMMC-7721 cels were treated with 8-bromo-7-methoxychrysin, sorafenib alone and their combination for 24 hours. Then, flow cytometry was used to detect cel apoptosis and western blot assay was used to determine nuclear factor-κB protein expression. RESULTS AND CONCLUSION:Compared with 8-bromo-7-methoxychrysin group and sorafenib group, the apoptotic rates of SMMC-7721 cels were significantly enhanced after treatment with the combination of 8-bromo-7-methoxychrysin group and sorafenib (2.5, 5, 10, 50 μmol/L), and meanwhile, the protein expression of nuclear factor-κB was down-regulated significantly. These findings indicate that the combined therapy of 8-bromo-7-methoxychrysin group and sorafenib can enhance the apoptosis of SMMC-7721 cels, which may be associated with down-regulation of nuclear factor-κB protein.
