Development of human embryonic stem cell platforms for human health-safety evaluation
10.3969/j.issn.1671-167X.2016.01.001
- VernacularTitle:基于人胚胎干细胞健康安全评价体系的构建
- Author:
Guangyan YU
;
Tong CAO
;
Xiaohui ZOU
;
Xuehui ZHANG
;
Xin FU
;
Shuangqing PENG
;
Xuliang DENG
;
Shenglin LI
;
He LIU
;
Ran XIAO
;
Hongwei OUYANG
;
Hui PENG
;
Xiao CHEN
;
Zengming ZHAO
;
Xiaoying WANG
;
Haiqin FANG
;
Lu LU
;
Yulan REN
;
Mingming XU
- Publication Type:Journal Article
- Keywords:
Embryonic stem cells;
Toxicity tests;
Cell differentiation;
In vitro;
Humans
- From:
Journal of Peking University(Health Sciences)
2016;48(1):1-4
- CountryChina
- Language:Chinese
-
Abstract:
SUMMARY Thehumanembryonicstemcells(hESCs)serveasaself-renewable,genetically-healthy, pluripotent and single source of all body cells,tissues and organs.Therefore,it is considered as the good standard for all human stem cells by US,Europe and international authorities.In this study,the standard and healthy human mesenchymal progenitors,ligament tissues,cardiomyocytes,keratinocytes,primary neurons,fibroblasts,and salivary serous cells were differentiated from hESCs.The human cellular health-safety of NaF,retinoic acid,5-fluorouracil,dexamethasone,penicillin G,adriamycin,lead ace-tate PbAc,bisphenol A-biglycidyl methacrylate (Bis-GMA)were evaluated selectively on the standar-dized platforms of hESCs,hESCs-derived cardiomyocytes,keratinocytes,primary neurons,and fibro-blasts.The evaluations were compared with those on the currently most adopted cellular platforms.Parti-cularly,the sensitivity difference of PM2.5 toxicity on standardized and healthy hESCs derived fibroblasts, currently adopted immortalized human bronchial epithelial cells Beas-2B and human umbilical vein endo-thelial cells (HUVECs)were evaluated.The results showed that the standardized hESCs cellular plat-forms provided more sensitivity and accuracy for human cellular health-safety evaluation.
