Effect of astragalus polysaccharides on the neuronal differentiation of bone marrow mesenchymal stem cells
10.3969/j.issn.2095-4344.2015.45.008
- VernacularTitle:骨髓间充质干细胞向神经元样细胞分化中黄芪多糖的诱导作用
- Author:
Guan WANG
;
Jingya LI
;
Shengping YANG
;
Jianmin WANG
- Publication Type:Journal Article
- Keywords:
Bone Marrow;
Mesenchymal Stem Cels;
Fibroblast Growth Factor 2;
Astragalus membranaceus;
Cel Differentiation;
Tissue Engineering
- From:
Chinese Journal of Tissue Engineering Research
2015;(45):7259-7262
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Active components of Astragalus have an antioxidant effect, which is considered to result in the neuron-like differentiation of bone marrow mesenchymal stem cels. OBJECTIVE:To investigate the effect of astragalus polysaccharides combined with basic fibroblast growth factor to induce the differentiation of bone marrow mesenchymal stem cels into nerve cels. METHODS:After 24 hours of pretreatment with basic fibroblast growth factor, passage 3 human bone marrow mesenchymal stem cels were cultured with astragalus polysaccharides for 1-3 days (combined group). Blank control group and basic fibroblast growth factor group were set up. Expression of neuron-specific enolase and nestin was detected using western blot or immunocytochemical staining. RESULTS AND CONCLUSION:The expression of neuron-specific enolase was higher in the combined group than the basic fibroblast growth factor group (P < 0.05). Expression of nestin was found in both basic fibroblast growth factor group and combined group, but the gray value was higher in the combined group than the basic fibroblast growth factor group (P < 0.05). These findings indicate that astragalus polysaccharides combined with basic fibroblast growth factor is better to induce the neuronal differentiation of bone marrow mesenchymal stem cels.
