Changes of pH values of N,O-CMC/β-TCP with different mass fractions in simulated body fluid degradation and their influence in growth of MG63 cells
10.13481/j.1671-587x.20140416
- VernacularTitle:不同质量分数N，O-CMC／β-TCP复合材料在模拟体液降解中的pH值变化及其对MG63细胞生长的影响
- Author:
Yuyan LIU
;
Dongsheng YU
;
Xingfu BAO
;
Shang GAO
;
Li SHA
;
Min HU
- Publication Type:Journal Article
- Keywords:
N,O-carboxymethyl chitosan;
β-tricalcium phosphate;
simulated body fluid;
methyl thiazoly tetrazolium
- From:
Journal of Jilin University(Medicine Edition)
2014;(4):772-776
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the changes of PH values of N,O-CMC/β-TCP compositive materials with different mass fractions in simulated body fluids (SBF)and their influence in the growth of MG63 cells, and to illustrate their mechanisms, and to provide reference for the further research on the bone repair materials. Methods The N,O-CMC/β-TCP with mass fractions of 2/1,1/1 and 1/2 were used as experimental groups,and the collagen nano calcium phosphate bone repair material as control group. The materials with different mass fractions were immersed in SBF and the pH values were measured by pH meter after soaking for 7,14,21 and 28d,respectively.The MG63 cells with the concentration of 1 × 105 mL-1 were inoculated and co-cultured in experimental and control groups,the adhesion and morphological changes of MG63 cells in each group were observed by scanning electron microscope and the cell proliferation was detected by MTT method after co-culturing for 2,4 and 6 d.Results The pH values were 6.70-7.25 in N,O-CMC/β-TCP (1/2)group and N,O-CMC/β-TCP (2/1)groups and the pH value in N,O-CMC/β-TCP (1/1)group was basically 7.15. The cells in N,O-CMC/β-TCP (2/1)group formed owe full,spreading face small and less secretion,but the cells in N,O-CMC/β-TCP 1/2 and 1/1 groups formed in full, pseudopodia interconnection, widely spreading and more secretions under electron microscope. The proliferation rate of the cells in N,O-CMC/β-TCP with (1/1 ) and N,O-CMC/β-TCP (2/1)groups had no statistical difference compared with control group (P>0.05),but there was significant difference between control group and N,O-CMC/β-TCP (1/2)group (P<0.05).Conclusion The changes of pH values of N,O-CMC/β-TCP materials with different mass fractions in SBF are small and the pH values are neutral;the order of the mass fraction of N,O-CMC/β-TCP to promote the growth of MG63 cells is 1/1,2/1,and 1/2.
