Regulation of Transforming Growth Factor -beta 1/Smad3 Signaling Pathway of Asthmatic Rat Airway Remodeling Model by Back Three-acupoint Electroacupuncture
10.13359/j.cnki.gzxbtcm.2015.01.016
- VernacularTitle:电针背三针对哮喘大鼠气道重塑模型TGF－β1／Smad3信号通路的调控
- Author:
Zheng LI
;
Pengdong JI
;
Yayun WANG
;
Xiaojie LIN
;
Zhenyi CHEN
;
Xiaozhe LI
;
Xinsheng LAI
- Publication Type:Journal Article
- Keywords:
Airway remodeling;
Asthma /acup-moxibustion therapy;
Lung/pathology;
Gene expression regulation;
Protein expression;
Disease models,animal;
Rats
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2015;(1):71-75
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of electroacupuncture on back three-acupoints ( namely bilateral Dazhu, Fengmen, Feishu) on the protein and mRNA expression of transforming growth factor-beta 1 (TGF-β1) and Smad3 in asthmatic rat airway remodeling model, and to explore its therapeutic efficacy and molecular mechanism. Methods Rat asthma model was established by inhalation of ovalbumin. After sensitization for 6 weeks, rats were killed. And then the airway morphological parameters of rats were measured by image analysis. The protein and mRNA expression of TGF-β1 and Smad3 in lung tissues were detected by immunohistochemistry and real-time quantitative polymerase chain reaction (qRT-PCR) respectively. Results Compared with the blank group, the standardized values of muscle cross-sectional area including airway smooth muscle area (WAm) /perimeter of the basement membrane (Pbm), and bronchial inner wall area (WAi)/Pbm were increased in the model group. The protein and mRNA expression levels of TGF-β1 and Smad3 were also increased in the model group. In electroacupuncture group, the above observation indexes were decreased, and the differences were statistically significant ( P<0.05 compared with the model group). Conclusion The experimental results indicated that back three-acupoint electroacupuncture has an effect on remodeling airway, and one of the mechanisms is probably associated with the regulation of TGF-β1/Smad3 signaling pathway.
