Effect of Jiaotai Pills on Hypothalamic Orexin A and Gamma-aminobutyric Acid in Sleep Deprivation Rats
10.13359/j.cnki.gzxbtcm.2015.01.022
- VernacularTitle:交泰丸对睡眠剥夺大鼠下丘脑Orexin A及γ-氨基丁酸的影响
- Author:
Shijian QUAN
;
Mengmeng JIAO
;
Shangyan HEI
;
Lili QIAN
- Publication Type:Journal Article
- Keywords:
Jiaotai Pills/pharmacology;
Central neurotransmitter;
Insomnia/TCD therapy;
Disease models,animal;
Rats
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2015;(1):103-105
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of Jiaotai Pills ( JP) on hypothalamic neurotransmitters of Orexin A and gamma-aminobutyric acid ( GABA) in rapid eye movement ( REM) sleep deprivation rats. Methods Rat model of REM-sleep deprivation was established by water small platform method. The rats were randomized into 6 groups, namely normal control group, model group, Diazepam group (3 mg/kg), and high-, medium-and low-dose JP groups ( JP in the dosage of 18.6, 9.3 and 4.6 g/kg respectively) . Enzyme-labeled instrument was used to detect the absorbance ratio of rat hypothalamic Orexin A content, and high performance liquid phase electrochemical detection method was adopted for the detection of hypothalamic GABA content. Results Compared with the normal control group, all of the rats in the model group suffered from insomnia, and the Orexin A content was increased ( P<0.05) . Compared with the model group, insomnia in rats of high-, medium- and low-dose JP groups was markedly relieved, and Orexin A was decreased (P<0.05) . The difference of GABA was insignificant between model group and JP groups (P>0.05) . Conclusion The sedative and hypnic mechanism of JP is probably related with the inhibition of hypothalamic Orexin A.
