Investigation of Protective Effect of Phenethyl Alcohol Glycosides Extracted from Herba Cistanchis on Human Sperm DNA with Oxidative Damage by Raman Spectroscopy

VernacularTitle:基于拉曼光谱技术的肉苁蓉苯乙醇苷对氧化损伤人精子DNA的保护作用
Author: Hualun LIANG ; Xiujuan JIANG ; Ben LI ; Diling CHEN
Publication Type:Journal Article
Keywords: Boschnaloside/pharmacology; Infertility/TCD therapy; Raman spectrum; Sperms; Anti-oxidative damage
From: Journal of Guangzhou University of Traditional Chinese Medicine 2015;(1):121-125
CountryChina
Language:Chinese
Abstract: Objective To study the protective effect of phenethyl alcohol glycosides extracted from Herba Cistan-chis on human sperm DNA with oxidative damage by Raman spectroscopy. Methods The human sperm model of oxidative damage was induced with Fenton’s reagent in vitro. After co-cultured with the phenethyl alcohol gly-cosides extracted from Herba Cistanchis ( in the dosage of 0.1, 0.01 and 0.001 μg/mL) , the changes of the sperm nuclear DNA were observed by using confocal micro-Raman spectroscopy. Results The intensity and peaks of the Raman spectra of the human sperm nuclei treated by Fenton’s reagent were changed significantly, and then the changes of intensity and peaks were inhibited after treatment with phenethyl alcohol glycosides of Herba Cistanchis, the inhibition being dose-dependent. Conclusion The phenylethyl alcohol glycosides ex-tracted from Herba Cistanchis have protective effect on human sperm DNA with oxidative damage.