Mechanism by which dihydroartemisinin inhibits invasion and migration of glioma stem cells
10.3969/j.issn.2095-4344.2016.06.001
- VernacularTitle:双氢青蒿素抑制胶质瘤干细胞侵袭迁移的机制
- Author:
Yanlin WU
;
Zheng CAI
;
Mingzhi ZHANG
;
Xiaorui FU
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2016;20(6):765-770
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Dihydroartmisinin can promote apoptosis of glioma cels GL261, but its effect on glioma stem cels is stil unknown. OBJECTIVE:To investigate the preliminary mechanism that dihydroartemisinin inhibits migration and invasion of glioma stem cels. METHODS: Glioma stem cels were isolated from mouse malignant glioma cel lines GL261. Immunofluorescence analysis was conducted to identify the characteristics of glioma stem cels. Migration and invasion abilities of glioma stem cels were analyzed by Transwel assay. The mRNA expressions of Tol-like receptor 2, matrix metaloproteinase-2 and matrix metaloproteinase-9 were examined by real-time fluorescence quantitative PCR. RESULTS AND CONCLUSION:The characteristics of glioma stem cels were identified by CD133 and Nestin staining. The migration and invasion of glioma stem cels were attenuated by dihydroartemisinin dose-dependently. Moreover, the mRNA expression of Tol-like receptor 2, matrix metaloproteinase-2 and matrix metaloproteinase-9 was also decreased by dihydroartemisinin in a dose dependent manner. These results suggest that dihydroartemisinin inhibits the migration and invasion of glioma stem cels probably through attenuation of Tol-like receptor signaling pathway.
