Correlation of peripheral blood bilirubin level with Tregs in patients with bile duct stones
10.3760/cma.j.issn.1007-8118.2016.04.009
- VernacularTitle:胆管结石患者外周血胆红素水平与调节性T淋巴细胞变化的相关性
- Author:
Hang LIU
;
Weimin LI
;
Haorun LIU
;
Ruiguan WANG
;
Hongqiang ZHAO
;
Yizhen WANG
- Publication Type:Journal Article
- Keywords:
Bile duct stone;
Jaundice;
Bilirubin;
Tregs
- From:
Chinese Journal of Hepatobiliary Surgery
2016;22(4):253-257
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study and correlate serum bilirubin and regulatory T cell (Treg) levels in patients with bile duct stone.Methods Flow-cytometry and Enzyme-linked immunosorbent assay (Elisa) were used to study the peripheral blood expression level of Tregs and the bilirubin level in 27 patients with bile duct stones and jaundice.The changes in the expression level of Tregs and the bilirubin level were studied and correlated before and after treatment.Results After treatment,both the peripheral blood bilirubin level,the Tregs expression level and the cell cytokines decreased significantly.The total bilirubin level decreased from (102.8 ± 33.1) mmol/L to (15.3 ± 5.7) mmol/L (P ＜ 0.05),the direct bilirubin level decreased from (38.1 ± 12.8) mmol/L to (5.0 ± 1.6) mmol/L (P ＜0.05);the percentages of CD4+ CD25 +Foxp3 + T cells in CD4+ T decreased from (4.2 ± 2.0) ％ to (2.4 ± 1.0) ％ (P ＜ 0.05).Before treatment,the levels of IL-10 and TGF-β were 171.4 ± 13.7 and 2016 ±657 pg/ml but after treatment,the two cytokines decreased to 92.1 ± 7.4 and 1 686 ± 168 pg/ml,respectively (P ＜ 0.05).Conclusions Patients with bile duct stones and jaundice presented with high expressions of bilirubin and Tregs level.These expressions returned to normal after effective treatment.The Tregs expression level was positively correlated with the bilirubin level.
