Radiosensitizing effect of baicalin on human cervical carcinoma HeLa cells
10.3969/j.issn.1000-4718.2015.12.003
- VernacularTitle:黄岑苷对宫颈癌细胞株 HeLa 的放射增敏作用
- Author:
Shuang HUI
- Publication Type:Journal Article
- Keywords:
Baicalin;
HeLa cells;
Radiosensitization;
PI3K/Akt signaling pathway
- From:
Chinese Journal of Pathophysiology
2015;(12):2126-2129
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effect of baicalin on the radiosensitization of HeLa cells .METHODS:The cell activity was determined by MTT assay .The radiosensitivity of HeLa cells was detected by colony formation assay . The cell cycle was analyzed by flow cytometry .The protein levels of Akt , p-Akt, Bad and p-Bad were examined by Western blot.RESULTS:The cell growth of the HeLa cells was inhibited by baicalin dose-dependently and IC 50 was 43.65 mg/L. The results of colony formation assay showed that combination of 8 mg/L baicalin and radiotherapy further improved survival curve and decreased the value of D0 and Dq, as compared with radiotherapy alone (P<0.05).Furthermore, baicalin en-hanced the effect of radiotherapy on cell cycle , as evidenced by the increase in cell percentage in G 2/M phase (P<0.05). Additionally, after incubation with baicalin , radiotherapy-induced phosphorylation of Akt and Bad were further augmented (P<0.05).CONCLUSION:Baicalin augments radiosensitivity of HeLa cells through the inhibition of cell cycle transi -tion and activation of PI3K/Akt signaling pathway.
