Effects of mitochondrial protectant on hippocampal mitochondrial function and synaptic plasticity in aged mice after isoflurane anesthesia
- VernacularTitle:线粒体保护剂对异氟醚麻醉老年小鼠海马线粒体功能和突触可塑性的影响
- Author:
Jing WU
;
Hui ZHANG
;
Xiaoru SUN
;
Jiaojiao YANG
;
Renqi LI
;
Jianjun YANG
- Publication Type:Journal Article
- Keywords:
Mitochondria;
Isoflurane;
Synaptic plasticity;
Aging;
Mouse
- From:
The Journal of Clinical Anesthesiology
2015;(12):1211-1214
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of mitochondrial protectant on hippocampal mito-chondrial function and synaptic plasticity in isoflurane-anesthesia aged mice.Methods Forty-eight fif-teen-month-old male C57BL/6 mice were equally divided into 4 groups (n=12):oxygen+normal sa-line (group CN),oxygen+SS-31 (group CS),isoflurane+normal saline (group IN),and isoflurane+SS-31 (group IS).SS-31(5 mg/kg)or normal saline was intraperitoneally administered with a vol-ume of 0.4 ml/kg 30 min before gas inhalation.After two hours gas inhalation,the hippocampus was removed to determine complex Ⅰ-Ⅳ activities,adenosine triphosphate (ATP)and mitochondrial membrane potential (MMP)levels from isolated mitochondria,and measure the content of synapsin-1,PSD-95,NMDAR2A,NMDAR2B,CaMKⅡα and CaMKⅡβ.Results Compared with the group IN,complex Ⅰ activity,ATP and MMP levels were increased,synapsin-1 and PSD-95 were up-regu-lated,whereas NMDAR2B,CaMK Ⅱα and CaMK Ⅱβ were down-regulated in the group IS (P <0.05).No significant difference was observed in the complex Ⅱ-Ⅳ activities and NMDAR2A expres-sion.Conclusion Mitochondrial protectant SS-31 attenuates isoflurane-induced mitochondrial dysfunc-tion and neuron synaptic plasticity impairments in aged mice.
