The expression of GABAR1 and NMDAR2B in aged rat cerebral temporal lobe by propofol injection
- VernacularTitle:丙泊酚对老年大鼠脑颞叶 GABAR1和 NMDAR2B表达的影响
- Author:
Lin QIAO
;
Zhen XING
;
Wei ZHAO
;
Xinsheng WANG
- Publication Type:Journal Article
- Keywords:
Propofol;
Morris water maze;
GABAR1;
NMDAR2B;
Temporal lobe
- From:
The Journal of Clinical Anesthesiology
2015;(12):1228-1231
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the change of learning and memory quality and the expression change of GABAR1 and NMDAR2B in aged SD rat cerebral temporal lobe by propofol injection and discuss the influence that may be caused by propofol.Methods Aged male sd rats were randomly divided into three groups,each group of 20.Propofol intraperitoneal injection was implemented to groups P1 and P7 according 50 mg/kg and maintained anesthesia for 3 hours.The same dose of emulsion was given to control group. Morris water maze test was done in the first days and the seventh days after intraperitoneal injection.Then the left temporal lobe was gathered and the mRNA transcription and protein expression of GABAR1 and NMDAR2B were detected by FISH and Immunofluorescence technique.Results Compared with control group,the latent time of group P1 was significantly prolonged and the numbers of passing through the target frequency were decreased significantly (P <0.05).Group P7 had no significant differences.The mRNA transcription and protein expression of GABAR1 in group P1 were increased significantly and the mRNA transcription and protein expression of NMDAR2B in group P1 were decreased significantly (P <0.05 ). Group P7 had no significant differences.Conclusion The short-term memory of brain declined after propofol was used.This may be related to the function of temporal lobe.The mechanism may be related to the up regulation of GABAR1 and the down regulation of NMDAR2B simultaneously.
