Effects of Hui-hui Gan-song Yin on accumulation of extracellular matrix of glomerular mesangial cells induced by high glucose
- VernacularTitle:回回甘松饮含药血清对高糖诱导的肾小球系膜细胞外基质堆积的影响
- Author:
Yi NAN
;
Lijie MA
;
Ling YUAN
- Publication Type:Journal Article
- Keywords:
Hui-hui Gan-song Yin;
glomerular mesangial cell;
accumulation of extracellular matrix;
fibronectin;
ColⅠ;
ColⅣ
- From:
Chinese Journal of Biochemical Pharmaceutics
2015;(11):15-18
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of Hui-hui Gan-song Yin(HGY) on the accumulation of extracellular matrix of rat glomerular mesangial cells(MCs) induced by high glucose.Methods The 40 SD rats were randomly divided into normal control group(distilled water), glurenorm group(10 mg/kg), HGY high-dose group(10 g/kg) and HGY low-dose group(5 g/kg), 10 rats in each group.The rats in each group were treated with corresponding drugs, twice a day.After 3 days, the serum containing each drug were prepared to culture rat MCs in vitro.The MCs were divided into the normal control group( 10% serum of rats in normal control group ) , high glucose group ( 30 mmol/L glucose +10% serum of rats in normal control group), glurenorm group(30 mmol/L glucose+10% serum of rats in glurenorm group), HGY high-dose group(30 mmol/L glucose+10% serum of rats in HGY high-dose group) and HGY low-dose group(30 mmol/L glucose+10% serum of rats in HGY low-dose group).The fibronectin(FN), ColⅠand ColⅣ levels were detected by Western blot.Results Compared with normal control group, the expression of FN, ColⅠand ColⅣ in high glucose group increased(P<0.01).The HCY suppressed the protein expression of FN, ColⅠand ColⅣ significantly(P<0.05).Conclusion The serum containing HGY could suppressed protein expression of FN , ColⅠand ColⅣ and inhibit the accumulation of extracellular matrix of MCs induced by high glucose, which could protect glomerulus and delay the development of diabetic nephropathy.
