Determination of preservative in iron maltose syrup by HPLC
- VernacularTitle:HPLC法测定麦芽糖铁糖浆中防腐剂的含量
- Author:
Xuwen JIAO
;
Lei ZHANG
;
Hua CHEN
- Publication Type:Journal Article
- Keywords:
iron maltose syrup;
methyl parahydroxybenzoate;
propyl parahydroxybenzoate;
determination
- From:
Chinese Journal of Biochemical Pharmaceutics
2015;(11):169-171
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a HPLC method for the determination of preservative in iron maltose syrup.Methods A Kromasil 100-5 C18 column was used with acetonitrile-sodium acetate buffer solution(40 ︰60) as the mobile phase at the flow rate of 1.0 mL/min and 254 nm as the detection wavelength.The column temperature was set at 30 ℃.Results The calibration curve was linear within the range of 0.62 ~3.72 μg/mL for methyl parahydroxybenzoate, 0.18~1.07μg/mL for propyl parahydroxybenzoate, and the linear equation was Y=228494X-2512.5,Y=203351X-3471.4, respectively.The average recovery of methyl parahydroxybenzoate, propyl parahydroxybenzoate was 100.9%(RSD=1.5%),99.6%(RSD=0.5%), respectively.Conclusion The established method is simple, rapid and accurate, which could be used in the determination of preservative in iron maltose syrup.
