Determination of Vancomycin and Norvancomycin in human serum by HPLC
- VernacularTitle:HPLC法测患者血清万古霉素和去甲万古霉素浓度
- Author:
Bin LIU
;
Tong TONG
- Publication Type:Journal Article
- Keywords:
HPLC;
Vancomycin;
Norvancomycin;
human serum;
plasma concentration
- From:
Chinese Journal of Biochemical Pharmaceutics
2015;(11):172-174
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish HPLC method for fast, effective, simultaneous determination of Vancomycin and Norvancomycin in serum. Methods The separation was achieved on the Hypersil ODS2(250 mm ×4.6 mm,5μm)with methanol-acetonitrile-a mixture of potassium phosphate (9.5:2.5:88) as the mobile phase.The flow rate was 1.0 mL/min.The UV detecting wavelength was 280 nm.The temperature of the column was 35 ℃. Vancomycin and Norvancomycin were used as the internal standard for each other .Results Vancomycin and Norvancomycin blood concentration were in well linearity in the range of 1.25-100μg/mL,R2 =0.9996.The average method recovery rate of three concentrations were 96.88%,99.50%,105.01%respectively, the average method recovery rate of three concentrations were 94.40%,103.90,103.81%respectively, the intra-day and inter-day RSD were less than 5%.Conclusion The HPLC method is proved to be simple,sensitive, accurate and precise in which little blood sample is taken, thus it is suitable for the therapeutic drug monitoring(TDM) and pharmacokinetic study of Vancomycin and Norvancomycin.
