Determination of ammonium glycyrrhizinate in Shufei Mixture by RP-HPLC
- VernacularTitle:反相高效液相色谱法测定舒肺合剂中甘草酸铵的含量
- Author:
Xuedan WU
;
Yanping JI
;
Jing DU
;
Yinghua FU
- Publication Type:Journal Article
- Keywords:
RP-HPLC;
ammonium glycyrrhizinate;
Shufei Mixture;
content determination
- From:
Chinese Journal of Biochemical Pharmaceutics
2015;(11):175-177
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a RP-HPLC method for determination of ammonium glycyrrhizinate in Shufei Mixture .Methods The sample was analyzed on an Agilent Zorbax SB-C18 column (4.6mm ×150 mm, 5μm), and acetonitrile-0.0025 mol/L sodium heptanesulfonate solution–0.05 mol/L potassium dihydrogen phosphate solution(20:45:45) ( adjusted pH value of 7.2 ±0.05 with 20% sodium hydroxide solution) was used as mobile phase.The flow rate was at 1.0 mL/min.The detective wavelength was at 250 nm.The column temperature was 30 ℃.Results With this chromatographic condition, the ammonium glycyrrhizinate peak in Shufei Mixture sample chromatogram could be separated with other ingredient peaks completely.The negative sample had no interference.The calibration curve was linear at a ranges of 23.6-118.1μg/mL for ammonium glycyrrhizinate, and equation of regression was Y=0.1133X–0.00110,r=0.999 8.The average recovery from sample was 97.8% and RSD was 0.88%(n=6).The content range of ammonium glycyrrhizinate in three batch Shufei Mixture sample was 0.2532-0.2865 mg/mL, and average content was 0.2721 mg/mL. Conclusion This method is simple, accurate, and useful for control method of this preparation.
