Different components of chicken egg-white extracts affect cell cycle and apoptosis
10.3969/j.issn.2095-4344.2015.46.014
- VernacularTitle:鸡卵清提取液不同成分对细胞周期与细胞凋亡的影响
- Author:
Guangping RUAN
;
Xiang YAO
;
Jufen LIU
;
Jinxiang WANG
;
Rongqing PANG
;
Xinghua PAN
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2015;(46):7451-7455
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:If an extract can prolong the S phase and reduce the percentage of apoptosis after co-culture with cels, it is proved that the extract is able to promote cel proliferation. OBJECTIVE: To prove the effects of chicken egg-white extracts with different components on the proliferation, cel cycle and apoptosis of 293T cels. METHODS: An ultrafiltration unit was used to separate chicken egg-white extracts into different components that were > 10 ku, < 10 ku, > 3 ku and < 3 ku to co-culture with cels for 3 days. Then, cel proliferation, cel cycle and cel apoptosis were detected. RESULTS AND CONCLUSION:Chicken egg-white extract components of < 10 ku and < 3 ku could promote cel proliferation, increase the percentage of S-phase cels and reduce the percentage of apoptosis. In conclusion, chicken egg-white extract components that are < 10 ku and < 3 ku can promote cel proliferation, as wel as increase the percentage of S-phase cels and reduce apoptosis percentage.
