Effect of silk fibroin/hydroxyapatite scaffold on the viability and osteogenic properties of adipose-derived stem cells under osteogenic induction
10.3969/j.issn.2095-4344.2015.45.002
- VernacularTitle:丝素蛋白/羟基磷灰石支架对成骨诱导脂肪干细胞活性及成骨性能的影响
- Author:
Hao LIU
;
Yawei CHU
;
Tao DING
;
Li CHENG
;
Haoming ZHU
- Publication Type:Journal Article
- Keywords:
Silk;
Hydroxyapatites;
Stem Cels;
Adipose Tissue;
Biocompatible Materials;
Tissue Engineering
- From:
Chinese Journal of Tissue Engineering Research
2015;(45):7224-7229
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Adipose-derived stem cels under osteogenic induction can be combined with biodegradable silk fibroin/hydroxyapatite scaffold, which is expected to develop a new biocompatible and osteogenic bone fusion material. OBJECTIVE:To study the effect of silk fibroin/hydroxyapatite composite on the viability and osteogenic properties of adipose-derived stem cels after osteogenic induction. METHODS:Adipose-derived stem cels were obtained from rat’s fat tissue, then adherently cultured, proliferated and passaged in vitro. Passage 3 cels were cultured in conditioned medium for osteogenic induction, and then seeded onto silk fibroin/hydroxyapatite scaffold as experimental group. Adipose-derived stem cels cultured on the cover glasses at the same condition acted as control group. The celular morphology, proliferation and differentiation were assessed respectively by means of phase contrast microscope, MTT assay and alkaline phosphatase activity measurement. RESULTS AND CONCLUSION:After osteogenic induction, adipose-derived stem cels could adhere to the scaffold material and proliferate on the surface of silk fibroin/hydroxyapatite scaffold normaly. No significant difference was found in cel proliferation and alkaline phosphatase activity between the experimental and control groups (P > 0.05), suggesting the celular activity and function were not affected by the material. These findings indicate that silk fibroin/hydroxyapatite composite material has good cytocompatibility. Subject headings: Silk; Hydroxyapatites; Stem Cels; Adipose Tissue; Biocompatible Materials; Tissue Engineering.
