Kallikrein 1-transfected bone marrow mesenchymal stem cells：selection of the multiplicity of infection
10.3969/j.issn.2095-4344.2015.45.006
- VernacularTitle:组织激肽释放酶1转染骨髓间充质干细胞的病毒感染复数选择
- Author:
Jia JIA
;
Limei JIN
;
Yi ZHAO
;
Li YAN
;
Juan LU
;
Qiuping WANG
;
Jingmiao ZHAO
;
Jihong HU
- Publication Type:Journal Article
- Keywords:
Tissue Engineering;
Stem Cels;
Transfection;
Green Fluorescent Proteins
- From:
Chinese Journal of Tissue Engineering Research
2015;(45):7249-7253
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Kalikrein 1 is an important component of the kalikrein-kinin system. Studies have shown that kalikrein can protect the cardiovascular system by promoting angiogenesis and inhibiting myocardial inflammation, but there is no report on its effect on inducing differentiation of stem cels. OBJECTIVE: To determine the transfection efficiency of kalikrein 1 adenoviral vector in rat bone mesenchymal stem cels. METHODS:Using adenovirus as a vector, the target gene kalikrein 1 was transfected into rat bone marrow mesenchymal stem cels. Fluorescence microscopy, MTT method and flow cytometry were used to investigate the effect of transfection and determine the optimal multiplicity of infection. RESULTS AND CONCLUSION:Adenovirus carrying kalikrein 1 was successfuly transfected into rat bone marrow mesenchymal stem cels. Results from flow cytometry showed that the transfection efficiency was associated with the multiplicity of infection. When the multiplicity of infection was 150, the transfection efficiency was 80.8%. MTT results showed that when the multiplicity of infection was 200, the cel growth was inhibited remarkably. These findings indicate that adenovirus-mediated kalikrein 1 can be successfuly transfected into rat bone marrow mesenchymal stem cels with the optimal multiplicity of infection=150.
