Differentiation of neural stem cells induced by erythropoietin in vitro
10.3969/j.issn.2095-4344.2015.45.022
- VernacularTitle:促红细胞生成素体外诱导神经干细胞的分化
- Author:
Yan ZHAO
;
Xiliang WANG
;
Yulong XIAO
;
Hongjun HUO
;
Jianming JIANG
;
Huibo YAN
- Publication Type:Journal Article
- Keywords:
Erythropoietin;
Neural Stem Cels;
Cel Differentiation;
Tissue Engineering
- From:
Chinese Journal of Tissue Engineering Research
2015;(45):7337-7341
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:In recent years, neural stem cels are considered to be ideal for the treatment of spinal cord injury, but the proportion of its natural differentiation into neurons in the host body is relatively low, which severely restricts the therapeutic effect on spinal cord injury. OBJECTIVE:To investigate the effect of erythropoietin on the differentiation of neural stem cels in vitro. METHODS:Under sterile condition, neural stem cels from the hippocampus of neonatal Wistar rats were isolated, cultured and identified by immunofluorescencein vitro. The third generation of neural stem cels were randomly divided into 0.5, 5, 50 U/mL erythropoietin groups and control group (with no erythropoietin). RESULTS AND CONCLUSION:Compared with the control group, the differentiation rate of neural stem cels was significantly improved in the 0.5, 5, 50 U/mL erythropoietin groups (P< 0.05); moreover, the differentiation rate of neural stem cels in the 5, 50 U/mL erythropoietin groups was higher than that in the 0.5 U/mL erythropoietin group (P < 0.05). In addition, there was no significant difference between the 5, 50 U/mL erythropoietin groups (P > 0.05). These findings indicate that erythropoietin can effectively induce the differentiation of neural stem cels into neurons in vitro, and moreover, it can significantly improve the differentiation rate of neural stem cels into neurons.
