Effectiveness of mineralized gelatin electrostatic spinning on inducing periodontal tissue osteogenesis
10.3969/j.issn.2095-4344.2015.47.007
- VernacularTitle:矿化明胶静电纺丝诱导牙周组织成骨的有效性
- Author:
Ruizheng GUO
;
Yali WANG
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2015;(47):7583-7588
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Currently, little is reported regarding the effectiveness of mineralized gelatin electrostatic spinning fibers on periodontal tissue osteogenic induction. OBJECTIVE: To investigate the effect of mineralized gelatin electrostatic spinning on the proliferation and osteogenic differentiation of periodontal ligament fibroblasts. METHODS: Human periodontal ligament fibroblasts were respectively compositely cultured with non-mineralized gelatin electrostatic spinning, gelatin electrostatic spinning after 1 day of nano-hydroxyapatite mineralization and gelatin electrostatic spinning after 5 days of nano-hydroxyapatite mineralization. Cel proliferation was determined at 1, 4, 7, 10 and 13 days of culture using MTT assay. Alkaline phosphatase activity of cels was determined at 1, 7, 14 days of culture using biochemical analyzer. RESULTS AND CONCLUSION:At the 10th day of culture, the periodontal ligament cels on the surface of membrane distributed uniformly, grew wel, spread out as sheets and secreted a large amount of extracelular matrix, linked closely with the materialsl; moreover, the effect was more obvious at 5 days of mineralization. Cel growth density and status were better than those in the non-mineralized gelatin electrostatic spinning membrane group. Cel proliferation and alkaline phosphatase activities at different time points: 5-day mineralized gelatin electrostatic spinning membrane group > 1-day mineralized gelatin electrostatic spinning membrane group > non-mineralized gelatin electrostatic spinning fibers group (alP < 0.05). These results demonstrate that the gelatin electrostatic spinning after nano-hydroxyapatite mineralization may promote the proliferation and osteogenic differentiation of periodontal ligament fibroblasts in a time-dependent manner.
