Bone marrow mesenchymal stem cells combined with chitosan for articular cartilage injuries
10.3969/j.issn.2095-4344.2015.45.007
- VernacularTitle:骨髓间充质干细胞与壳聚糖复合修复关节软骨损伤
- Author:
Lu CHEN
;
Xianxue XIA
;
Ke JIANG
- Publication Type:Journal Article
- Keywords:
Bone Marrow;
Mesenchymal Stem Cels;
Chitosan;
Cartilage,Articular;
Tissue Engineering
- From:
Chinese Journal of Tissue Engineering Research
2015;(45):7254-7258
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Trauma easily leads to the emergence of articular cartilage defects, which is a difficult problem in the orthopedics field. Tissue engineering technology provides a new method for cartilage repair. OBJECTIVE:To explore the feasibility of combining chitosan and bone marrow mesenchymal stem cels to repair injured articular cartilage in rabbits. METHODS: Cultured rabbit bone marrow mesenchymal stem cels were seeded onto chitosan scaffold, and then the composite material was implanted into the defect as experimental group. Rabbits with no treatment served as control group. Gross observation and toluidine blue staining were carried out at 6 and 12 weeks after operation. RESULTS AND CONCLUSION:At 6 weeks after operation, the control group had only fibrous tissue hyperplasia, and in the experimental group, cartilage-like tissues were generated at the defect site. At 12 weeks after operation, a smal amount of hyaline cartilage-like tissues were observed in the control group, and the defects in the experimental group were covered with smooth and hyaline cartilage tissues. After 12 weeks, the toluidine blue staining was light in the control group with a smal amount of cartilage tissues; in the experimental group, the toluidine blue staining was remarkable, and the defects were completely covered with hyaline cartilage tissues, and cartilage cels were increased in number. The findings indicate that chitosan-bone marrow mesenchymal stem cels composite material is better to induce cartilage tissue formation and promote cartilage defect repair.
