Regulation of endometrial stromal cells through the Wnt/beta-catenin signaling pathway
10.3969/j.issn.2095-4344.2016.07.014
- VernacularTitle:Wnt/β-catenin信号通路对子宫内膜间质细胞的调节机制
- Author:
Manru CHEN
;
Zhi LIAO
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2016;20(7):1013-1018
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Increasing number of genes and signaling pathways are involved in regulating stem cels periodicaly, and wherein Wnt/β-catenin signaling pathway is an important pathway of stem cels.
OBJECTIVE:To investigate the effects of Wnt/β-catenin signal pathway on mouse endometrial stromal cels.
METHODS:After injection of Wnt/β-catenin pathway inhibitor or activator, endometrial tissues from Balb/c mice were obtained, some of which were used for detection of invasion of endometrial stromal cels and western blot detection, and the rest of which were used for preparing animal models of endometriosis folowed by immunohistochemical detection.
RESULTS AND CONCLUSION:The expression of β-catenin and GSK3β proteins was significantly higher in the activator group than the inhibitor group (P < 0.05). The number of transmembrane cels was significantly higher in the activator group than the inhibitor and control groups (P < 0.05). Immunohistochemical findings showed positive expression of E-cadherin in ectopic endometrial tissues of the inhibitory group, and strongly positive expression of vascular endothelial growth factor in ectopic endometrial tissues of the activator group. These findings indicate that Wnt/β-catenin signaling pathway may cause endometriosis by strengthening ectopic endometrial implantation, invasion and metastasis.
