Comparison of the Anyplex II STI-7 and Seeplex STD6 ACE Detection Kits for the Detection of Sexually Transmitted Infections.
- Author:
Se Jin MOON
1
;
Jung Eun CHOI
;
Kwang Il PARK
Author Information
1. Department of Laboratory Medicine, U2Bio Laboratory, Seoul, Korea. moon8112sj@naver.com
- Publication Type:Original Article
- Keywords:
Sexually transmitted diseases;
Multiplex polymerase chain reaction;
Sexually transmitted infections-7
- MeSH:
Chlamydia trachomatis;
Methods;
Multiplex Polymerase Chain Reaction;
Mycoplasma genitalium;
Mycoplasma hominis;
Neisseria gonorrhoeae;
Prevalence;
Sexual Behavior;
Sexually Transmitted Diseases;
Trichomonas vaginalis;
Ureaplasma;
Ureaplasma urealyticum
- From:Journal of Laboratory Medicine and Quality Assurance
2013;35(2):87-92
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Sexually transmitted infections (STI) encompass a variety of clinical syndromes caused by many pathogens that are transmitted through sexual activity. Multiplex PCR is frequently used to detect STI. In this study, two multiplex real-time PCR-based assays were used to detect STI in clinical specimens, and the concordance of the results obtained by each method was evaluated. METHODS: A total of 626 specimens were tested using the Anyplex II STI-7 (Seegene, Korea) and Seeplex STD6 ACE Detection kits (Seegene). RESULTS: Among the 626 individuals tested, 227 (44.2%) tested positive for STI by using Anyplex II STI-7. The prevalence rates of the various infectious microorganisms detected were as follows: Chlamydia trachomatis (C. trachomatis), 19.2% (120/626); Neisseria gonorrhoeae (N. gonorrhoeae), 5.6% (35/626); Trichomonas vaginalis (T. vaginalis), 0.2% (1/626); Mycoplasma genitalium (M. genitalium), 8.1% (51/626); Mycoplasma hominis (M. hominis), 2.9% (18/626); Ureaplasma urealyticum (U. urealyticum), 17.6% (110/626); and Ureaplasma parvum, 3.7% (23/626). The concordance rates for the STI-7 and STD6 assays in detecting the various types of microorganism were as follows: C. trachomatis, (99.5%); N. gonorrhoeae, (99.7%); T. vaginalis, (100%); M. genitalium, (100%); M. hominis, (100%); and U. urealyticum (99.2%). CONCLUSIONS: A high degree of concordance was observed between the results obtained using the Anyplex II STI-7 kits and those obtained using the Seeplex STD6 ACE Detection kits.
