Human bone marrow mesenchymal stem cells promote epithelial mesenchymal transition in lung cancer cells
10.3969/j.issn.2095-4344.2016.07.011
- VernacularTitle:间充质干细胞条件培养液促进肺癌细胞上皮间质转化
- Author:
Jiabin WU
;
Tao WANG
;
Weilin YANG
;
Junjie WANG
;
Jiefei XIAO
;
Ruchen WANG
;
Zhenguang CHEN
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2016;20(7):993-999
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:The complex relationship between bone marrow mesenchymal stem cels and cancers severely limit the clinical application of mesenchymal stem cels. So it is urgent to study the role of mesenchymal stem cels in tumor growth and metastasis.
OBJECTIVE:To explore the effect of human bone marrow mesenchymal stem cels on epithelial mesenchymal transition in non-smal cel lung cancer A549 and PAa cels.
METHODS:The A549 and PAa cels were cultured with mesenchymal stem cel supernatant (mesenchymal stem cel conditioned medium, MSCs-CM). The celular morphology was observed under a microscope. The mRNA and protein expression of E-cadherin, N-cadherin, Vimentin, Slug, Snail, and Twist were determined by RT-PCR and western blot. Transwel and wound healing assay were used to detect the change of migration and metastatic ability.
RESULTS AND CONCLUSION:Compared with the control group, the celular morphology of experimental group showed mesenchymal-like changes. In response to MSCs-CM, there was decreased E-cadherin but increased N-cadherin, Vimentin and Slug, Snail, Twist at mRNA and protein levels compared with the control group (P < 0.05). The migration and metastatic abilities of the experimental group were also increased. So, human bone marrow mesenchymal stem cels can promote epithelial mesenchymal transition in A549 and PAa cels, and enhance the migration and metastatic abilities of A549 and PAa cels.
