Protective effect and mechanism ofShenfu injection on the oxidaitve damage in PC12 cells induced by H2O2
10.3760/cma.j.issn.1673-4246.2016.04.015
- VernacularTitle:参附注射液对过氧化氢诱导PC12细胞氧化损伤的保护作用及机制
- Author:
Yanni LYU
;
Longsheng FU
;
Hongwei PENG
;
Xiaochun SUN
;
Haili ZHONG
- Publication Type:Journal Article
- Keywords:
Shen Fu Zhu She Ye;
Oxidaitve damage;
Hydrogen peroxide;
PC12 cells;
NF-κB signaling pathway
- From:
International Journal of Traditional Chinese Medicine
2016;38(4):341-344
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects and probable mechanism ofShenfu injection on the oxidaitve damage of H2O2-induced PC12 cells.Methods PC12 cells were cultured and exposed to 100μmol/L H2O2 for 1 h to establish the oxidative damage model. The protective effect ofShenfu injection was observed by the cell survival rate measured by colorimetric MTT assay, and the leakage rate of lactic dehydrogense (LDH). Western blot methods were used to detect the expression of NF-κB signaling pathway.Results Compared with the model group,Shenfu injection at 5, 10, 20 ml/L could improve the PC12 cells survival rate (83.11% ± 2.59 %, 87.99% ± 0.59%, 85.26% ± 1.07%vs. 73.82% ± 1.82%;P<0.01 orP<0.05), decrease the LDH leakage rate (32.75% ± 4.10%, 28.52% ± 1.14%, 35.79% ± 1.62%vs. 64.34% ± 3.18%;P<0.01 or P<0.05). Western blot results showed thatShenfu injection could protect the PC12 cells from oxidaitve damage by suppressing the p-p65/p65 (1.30 ± 0.10, 1.17 ± 0.06, 1.37 ± 0.15 vs. 1.70 ± 0.10;P<0.01 orP<0.05), p-IκBα/IκBα (1.07 ± 0.12, 1.00 ± 0.10, 1.03 ± 0.06 vs. 1.17 ± 0.06; P<0.01 orP<0.05).ConclusionShenfu injection has a obvious antioxidant effect on PC12 cells in vitro.
