Using quadratic regression universal rotary combination design to optimize the fluorescence labeling condition of lycium barbarum polysaccharide
10.3760/cma.j.issn.1673-4246.2016.04.018
- VernacularTitle:二次回归通用旋转组合设计法优化枸杞多糖的荧光标记
- Author:
Huizhen CAI
;
Huishan WU
;
Xiaohui YANG
;
Binbin REN
- Publication Type:Journal Article
- Keywords:
Lycium barbarum polysaccharide;
Fluorescein isothiocyanate;
Tyramine;
Quadratic regression universal rotary combination design
- From:
International Journal of Traditional Chinese Medicine
2016;38(4):355-358
- CountryChina
- Language:Chinese
-
Abstract:
Objective Quadratic regression universal rotary combination design was used to optimize the fluorescence labeling condition of lycium barbarum polysaccharide (LBP).Methods The fluorescence labeling condition of LBP presented, when the covalent coupling of LBP and tyramine was reacted with fluorescein isothiocyanate (FITC). Filter the best labeling condition via using quadratic regression universal rotary combination design experiment on the relationship of labeling efficiency among pH value of buffer solution, reaction time, temperature and the dose of tyramine.Results The regression equation was:Y=0.085 41 - 0.002 82X1 - 0.015 68X2 + 0.008 11X3 + 0.005 01X4 + 0.008 75X1X2 - 0.005 75X1X3 - 0.001 75X1X4 + 0.010 63X2X3 + 0.000 125X2X4 + 0.000 25X3X4 - 0.021 44X12 - 0.008 89X22 - 0.001 984X32 + 0.003 66 X42, and the variables fromX1 toX4 represented pH value, reaction time, temperature and dose of tyramine, respectively. The goodness of fitting of regression equation was statistically significant. The condition of labling LBP was optimized when the temperature was at 50℃, timing of labling at the fourth day and pH value was 8.5.Conclusion The condition of labling LBP was optimized with suitable temperature, pH value and extended timing.
