Effects of epidermal growth factor on cell cycle and cell cycle-related regulatory factors of human esophageal squamous cell carcinoma cell line Eca109

VernacularTitle:表皮生长因子对食管鳞癌细胞Eca109细胞周期及其调控因子的影响
Author: Qianqian LI ; Hong ZHU ; Chaoli WANG ; Shijuan LI ; Weimin HU
Publication Type:Journal Article
Keywords: Epidermal growth factor; Esophageal squamous cell carcinoma; Eca109 cell line; Cell cycle; p21CIP1/WAF1; p27 KIP1
From: Chinese Journal of Immunology 2015;31(12):1616-1620
CountryChina
Language:Chinese
Abstract: Objective:To investigate the effects of epidermal growth factor (EGF)on cell cycle and cell cycle-related regulatory factors of human esophageal squamous cell carcinoma (ESCC) cell line Eca109.Methods: Serum starved Eca109 cells were treated with 20 ng/ml recombinant human EGF(rhEGF)for 24 h.The cell cycle phase distribution was detected by flow cytometry.The mRNA and protein expression levels of p21CIP1/WAF1(p21) and p27KIP1(p27) were detected by real-time quantitative reverse transcription polymerase chain reaction(qRT-PCR)and Western blot,respectively.Results: The proportions of G1 phase cells in EGF group and control group were ( 54.90 ±0.82 )% and ( 65.94 ±0.74 )%.The mRNA and protein expression levels of p 21 in EGF group was significantly higher ,and p27 was significantly lower than that in control group ( P<0.01 ) .Conclusion: EGF facilitates G1-S phase transition,and promotes the proliferation of Eca 109 cells,which may be associated with the up-regulation of p21 and down-regulation of p27.