Effects of acitretin and IFNα-2a on apoptosis and caspases expressions in human cutaneous squamous cell carcinoma cell line SCL-12
10.3969/j.issn.1000-484X.2015.12.009
- VernacularTitle:阿维A酸联合IFNα-2a对皮肤鳞状细胞癌细胞SCL-12凋亡及caspases影响
- Author:
Leilei PENG
;
Guoyong ZHOU
- Publication Type:Journal Article
- Keywords:
Acitretin;
IFNα-2a;
Squamous cell carcinoma;
Apoptosis
- From:
Chinese Journal of Immunology
2015;31(12):1624-1627
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effects of acitretin or IFNα-2a or both on the apoptosis and caspases expressions in human-cutaneous squamous cell carcinoma cell line SCL-12.Methods: SCL-12 cells were cultured in vitro in the presence of or acitretin or IFNα-2a or both.Apoptosis was detected by enzyme-linked immunosorbent assay(ELISA) in these cells.Apoptotic cells were observed by acridine orange staining .The protein expressions of caspase-3,caspase-8 and caspase-9 in SCL-12 cells were analyzed by Western blot.Results:The inhibition ratio of the cell growth significantly higher in groups of Acitretin or IFNα-2a or both than control group , and the effects of Acitretin combined with IFNα-2a stronger than the Acitretin or IFNα-2a.Caspase-3,caspase-8 and caspase-9 were significantly activated in combined group .The effects of Acitretin combined with IFNα-2a stronger than the Acitretin or IFNα-2a.Conclusion:Acitretin or IFNα-2a or both plays a central role in the apoptosis of cutaneous squamous cell carcinoma cells ,which may be related to the Fas-way and mitochondria-way.
