Study on Hydrolyzed Peptides of Red Cubilose and White Cubilose by HPLC-ESI-MS
10.11842/wst.2015.11.004
- VernacularTitle:HPLC-ESI-MS联用法分析血燕与白燕水解多肽研究
- Author:
Geng LI
;
Jie DAI
;
Ran ZHAO
;
Bin ZHAO
;
Minhua PENG
;
Xiaoping LAI
- Publication Type:Journal Article
- Keywords:
red cubilose;
white cubilose;
peptide map;
capillary high-performance liquid chromatography
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2015;(11):2202-2208
- CountryChina
- Language:Chinese
-
Abstract:
HPLC-ESI-MS technology was applied to determine the hydrolyzed peptides of red cubilose and white Abscubilose for the comparison of similarities and differences between different kinds of cubiloses' hydrolysis polypeptide primary structure.SDS-PAGE,capillary high-performance liquid chromatography and ESI-MS were used to detect the purity and peptide mapping of samples.Mascot 2.2 sof tware was used to search the database.The basepeak figure in mass spectrometry analysis showed good consistency of hydrolyzed peptides between red cubilose and white cubilose.The Mascot 2.2 sof tware was used to search the poultry database which had been downloaded from the UniProt.A total of 73 kinds of proteins from white cubilose were identified.And a total of 51 kinds of proteins from red cubilose were identified.There were 30 kinds of same proteins from both cubilose.It was concluded that the hydrolyzed peptides had relatively high similarity between red cubilose and white cubilose.The same proteins from both red and white cubilose can be used as the quality control characteristics of cubilose products.
