Determination on Human Plasma Protein Binding Rate of Ginkgolide A, B and K in Diterpene Ginkgolides Meglumine Injection
10.11842/wst.2015.11.009
- VernacularTitle:银杏二萜内酯葡胺注射液中银杏内酯A、B、K与人血浆蛋白结合率的测定
- Author:
Haihong SI
;
Ting GENG
;
Yanjing LI
;
Wenzhe HUANG
;
Zhenzhong WANG
;
Wei XIAO
- Publication Type:Journal Article
- Keywords:
Diterpene ginkgolides meglumine injection;
plasma protein binding rate;
balance dialysis;
LC-MS/MS
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2015;(11):2235-2239
- CountryChina
- Language:Chinese
-
Abstract:
This paper was aimed to study the human plasma protein binding rate of diterpene ginkgolides meglumine injection.The equilibrium dialysis was used to determine the human plasma protein binding rate of ginkgolide A (GA),ginkgolide B (GB) and ginkgolide K (GK) in diterpene ginkgolides meglumine injection.The LC-MS/MS method was used for the content determination of ginkgolides.And then,the plasma protein binding rate was calculated.The results showed that there was no interference from other ingredients for the determination of ginkgolides.The calibration curve of the analytes was in good linearity in certain range of contents.The precision and stability of the analytes met the methodology requirements.After 8 h incubation,the human plasma protein binding rate of GA,GB and GK achieved balance.The human plasma protein binding rate of GA (0.34,1.70 and 8.51μg·mL-1) was 84.03%-88.11%; the human plasma protein binding rate of GB (0.62,3.09 and 15.5μg·mL-1) was 41.21%-53.56%; the human plasma protein binding rate of GK (0.04,0.20 and 1.01μg·mL-1) was 45.24%-59.59%.It was concluded that the method was simple,rapid and sensitive,which met the analysis requirement for biological samples.GA had a high plasma protein binding rate; GB and GK had medium plasma protein binding rate.
