Optimization on Extraction Technology of Polysaccharide from Rhizoma Coptidis by Box-Behnken Design-Response Surface Methodology
10.11842/wst.2015.11.010
- VernacularTitle:Box-Behnken设计-效应面法优化黄连多糖的提取工艺
- Author:
Xiumei LV
;
Ce TANG
;
Gan FAN
;
Yan LI
;
Jing ZHANG
;
Yi ZHANG
- Publication Type:Journal Article
- Keywords:
Rhizoma Coptidis;
polysaccharides;
Box-Behnken design-response surface methodology;
extraction technology
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2015;(11):2240-2245
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to optimize the extraction conditions of polysaccharides from Rhizoma Coptidis.With the R.Coptidis of extraction yield,polysaccharide yield and uronic acid yield as evaluation indexes,the impact of extraction temperature,extraction times,extraction duration and liquid-to-solid ratio on the process of R.Coptidis polysaccharides reflux extraction were investigated by the Box-Behnken design-response surface methodology.The results showed that the optimal extraction conditions were achieved and listed as follows:extraction temperature at 100℃,extracted 3 times with 3.8 h per each time,liquid-to-solid ratio of 1:15.7.It was concluded that the Box-Behnken design-response surface methodology was accurate,rational and feasible to optimize the extraction method of polysaccharides fromR.Coptidis.
