Effects of Radix Paeoniae Alba Extract on Distribution and Expression of 5-HT3A/3B R in Frontal Lobe of Premenstrual Syndrome Rat Model with Liver-qi Depression Pattern
10.11842/wst.2015.11.014
- VernacularTitle:白芍提取物对经前期综合征肝气郁证模型大鼠额叶5-HT3A/3BR分布与蛋白表达的影响
- Author:
Fang LI
;
Chunhong SONG
;
Sheng WEI
;
Huiyun ZHANG
;
Mingqi QIAO
- Publication Type:Journal Article
- Keywords:
Radix Paeoniae Albaextract;
premenstrual syndrome;
liver-qi depression pattern;
frontal lobe;
5-HT3AR;
5-HT3BR;
protein expression
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2015;(11):2267-2271
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to elucidate the 5-HT3R molecular mechanism of Radix Paeoniae Albaextract (RPAE) as drug intervention in the treatment of premenstrual syndrome (PMS) model rats with liver-qi depression pattern.PMS model rats with liver-qi depression pattern were prepared.And then,the model was treated with RPAE.The protein distribution of 5-HT3AR and 5-HT3BR in the frontal lobe was evaluated by the immune fluorescence technology and western blot.The results showed that there were positive expressions of 5-HT3AR and 5-HT3BR in frontal lobe of rats in each group.Compared with the normal group,the 5-HT3AR and 5-HT3BR protein expression levels of the frontal lobe in the model group increased significantly (P < 0.05).Compared with the model group,the 5-HT3AR protein expression level in the frontal lobe decreased significantly after RPAE treatment (P < 0.05).In conclusion,RPAE regulated the protein expressions of 5-HT3AR and 5-HT3BR in frontal lobe,which may be one of the mechanisms for its treatment of PMS with liver-qi depression pattern.
