Ursolic Acid Inhibition on Human Hepatic Stellate Cells Proliferation through PDGF-ERK Signaling Pathway

VernacularTitle:乌索酸抑制人肝星状细胞增殖的PDGF-ERK信号通路研究
Author: Chunlin CHEN ; Yi ZHANG ; Qingyun BAI
Publication Type:Journal Article
Keywords: Ursolic acid; human hepatic stellate cells; cell proliferation; p-ERK1/2
From: World Science and Technology-Modernization of Traditional Chinese Medicine 2015;(11):2349-2353
CountryChina
Language:Chinese
Abstract: This study was aimed to investigate the effects of ursolic acid on human hepatic stellate cells (HSC-LX-2) proliferation and its mechanism.Different doses of ursolic acid were incubated with HSC-LX-2 cellin vitrof or 48 h.MTT was used for the detection of HSC-LX-2 cell proliferation.The expressions of PDGF-ERK signaling pathway associated proteins were measured by western blot.The results showed that the proliferation of HSC- LX-2 cells was inhibited by ursolic acid in a dose-dependent manner.The inhibition rate of 20,30 and 40μmol·L-1 of ursolic acid was 9.1%,42.3% and 62.6%,respectively.The IC50 was 35.2μmol·L-1.After incubated with ursolic acid for 48 h,protein levels of PDGF-R and p-ERK in 30 and 40μmol·L-1 group were significantly decreased when compared with the normal group (P<0.05 orP<0.01),except the ERK protein.It was concluded that ursolic acid can inhibit HSC-LX-2 cell proliferation.Its mechanism may be related to the blockage of PDGF-ERK signaling pathway.