Application of DNA-image cytometry in the diagnose of urothelial cell carcinomas

VernacularTitle:细胞DNA定量分析技术在尿路上皮癌诊断中的应用价值
Author: Jie SU ; Wei TAO ; Pei ZHANG ; Zhaozhao LIANG ; Jun LI
Publication Type:Journal Article
Keywords: Urothelial cell carcinomas; DNA ploidy; DNA-image cytometry; Liquid-based cytology
From: The Journal of Practical Medicine 2016;32(5):750-753
CountryChina
Language:Chinese
Abstract: Objective To investigate the value of DNA-image cyt-ometry (DNA-ICM) in the diagnosis of urothelial cell carcinomas (UCC). Methods Totally 162 voided urine specimens (92 cases from urothel-ial car-cinomas patients and 70 cases from benign urinary system diseases patients ) were detected with DNA-ICM and liquid-based cytology (LBC), respectively. Results The sensitivity and specificity of DNA-ICM were 65.2%and 100% respectively in the diagnosis of UCC but those of LBC were 27.2% and 98.6%, respectively. The sensitivity of DNA-ICM was significantly higher than that of LBC in the diagnosis of UCC (P < 0.01). The sen-sitivity of DNA-ICM in upper urinary tract urothelial cell carcinomas (UTUC) were 77.1%, which was much higher than that in bladder urothelial cell carcinomas (57.9%) but no statistical significance was found (P >0.01). Conclusion DNA-ICM, which improves the positive rate of urinary cytology, has great application value in the diagnosis of urothelial cell carcinomas and it is an effective screening method for urothelial cancer in diag-nosis and follow-up.