Influence of FOXC1 downregulating on biological behavior of nasopharyngeal carcinoma (NPC) 5-8F cell lines
10.3969/j.issn.1006-5725.2016.04.006
- VernacularTitle:下调叉头框转录因子C1对鼻咽癌5-8F细胞株生物学行为的影响
- Author:
Bolin CHEN
;
Shijiang YI
;
Peng LIU
;
Lei OUYANG
;
Xun LEI
- Publication Type:Journal Article
- Keywords:
FOXC1;
Nasopharyngeal carcinoma;
RNAi
- From:
The Journal of Practical Medicine
2016;32(4):527-530
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the Influence of FOXC1 downregulating on biological behavior of nasopharyngeal carcinoma (NPC) 5-8F cell lines. Methods Western blotting was used to detect the protein levels of FOXC1 in human immortalized nasopharyngeal cell lines NP69, nasopharyngeal carcinoma cell lines 6-10B with lower metastasis potential and nasopharyngeal carcinoma cell lines 5-8F with higher metastasis potential. siRNA was used to downregulate the expression of FOXC1 in 5-8F cell lines, and then ability of migration and invasion were observed. Results FOXC1 protein expression level in NP69 cell lines, 6-10B cell lines and 5-8F cell lines were (0.27 ± 0.04), (0.7 ± 20.06), (1.08 ± 0.05) respectively, and the difference was statistically significant (P < 0.05). Also ability of cell migration and invasion significantly weakened after FOXC1 depletion in 5-8F cell lines (P < 0.05). Conclusion FOXC1 might regulate invasion and metastasis of nasopharyngeal carcinoma through changing expression of Fibronectin and Vimentin, and FOXC1 may be an available target for molecular target therapy of nasopharyngeal carcinoma.
