Computerized optimization of high-performance liquid chromatographic method by using DryLab to test and isolate six cytochrome P450 probe substrates

VernacularTitle:DryLab软件辅助建立同时测定6种细胞色素P450酶探针底物的HPLC法
Author: Wenning YANG ; Yanli PAN ; Ruirui YANG ; Xiaoyun MA ; Xinjing CHEN ; Zhiqiang LUO ; Hao WANG ; Yang LIU
Publication Type:Journal Article
Keywords: Cytochrome P-450 enzyme system; High-performance liquid chromatographic; DryLab; Probe drug
From: International Journal of Traditional Chinese Medicine 2016;38(4):350-354
CountryChina
Language:Chinese
Abstract: Objective DryLab software was used to assist high performance liquid chromatography (HPLC) method to test and isolate six Cytochrome P450 (CYP450) probe substrates.Methods Six CYP450 probe substrates were selected and the right HPLC method was developed and validated with the assistance of DryLab software.Results The new HPLC method with the assistance of DryLab software could test and isolate six probe substrates with degrees of isolation more than 2.00. The correlation coefficients (R> 0.999 8) indicated high linear correlation between the concentrations and the peak areas among six probe substrates. Recovery studies showed good results for all the probe substrat from 86.38% to 110.29%. And therelative standard deviation (RSD) ranged from 1.69% to 3.80% with its intra-day and inter-day precision ranging from 0.42% to 2.01%, and 1.36% to 2.29%, respectively.Conclusions The developed HPLC method with the assistance of DryLab could test and isolate six probe substrates with shortertime than the HPLC method alone.