Laboratory diagnosis of rarely filamentous fungi
10.7644/j.issn.1674-9960.2015.11.011
- VernacularTitle:临床少见丝状真菌的实验室诊断
- Author:
Xiuyun YIN
;
Yuying LIANG
;
Nong YU
;
Lijun ZENG
;
Yihe LI
;
Qian JIANG
;
Jianmin XU
;
Zhe YANG
;
Haolian LI
;
Jiankui CHEN
;
Shuiping CHEN
- Publication Type:Journal Article
- Keywords:
rDNA;
filamentous fungi;
ITS2;
morphological identification;
sequence analysis
- From:
Military Medical Sciences
2015;(11):855-858
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the laboratory diagnosis of filamentous fungi which are rarely seen in clinical practice.Methods Five strains of fungi were isolated from clinical samples and initially identified by the morphological method.Total DNA of fungi was extracted and amplified by the PCR method using universal primers of ITS2-ITS4 gene, respectively.The PCR products were sequenced and the obtained sequences were then analyzed by the blastn program incorporated in NCBI.Results The five strains of fungi were diagnosed as Scedosporium apiospermum,Schizophyllum commune,Scopulariopsis brevicaulis,Rhizopus stolonifer,and Fusarium solani.Conclusion The laboratory diagnosis of filamentous fungi which rarely occur in clinical practice should integrate various methods,including morphological, microbiological,and molecular biological methods.
