Effect of Radix Bupleuri Extract on 5-HT3R Pathway in Primarily Cultured Rat Hippocampal Neurons
10.11842/wst.2015.11.007
- VernacularTitle:柴胡提取物对大鼠海马原代培养神经元5-HT3受体介导离子通道的影响
- Author:
Fang LI
;
Jieqiong WANG
;
Chunhong SONG
;
Sheng WEI
;
Huiyun ZHANG
;
Mingqi QIAO
- Publication Type:Journal Article
- Keywords:
Radix Bupleuri extract;
depression emotion model;
5-HT3R;
hippocampal neuron;
primary culture;
whole-cell patch clamp
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2015;(11):2224-2228
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to investigate the effect of Radix Bupleuri extract (RBE) on 5-HT3R channel currents of primarily cultured hippocampal neurons in depression emotion rats.Depression emotion model ratswere duplicated.RBE was used for drug intervention.And then,the rats were evaluated by the open-field test (OFT) and the sucrose preference test.Serum of rats in each group was collected and then added into the primary cultured hippocampal neurons for 24 h.The 5-HT3R channel currents were recorded by the whole-cell patch clamp.The results showed that compared with the normal group,the total score of OFT in the model group was significantly decreased (P < 0.01); the sucrose preference ratio decreased obviously (P < 0.01); and the current density value of primary cultured hippocampal neurons in serum of the model group was significantly higher (P <0.01).Compared with the model group,the total scores of OFT in the RBE group and fluoxetine group increased significantly (P < 0.05,P < 0.01); the sucrose preference ratio also increased obviously (P < 0.05,P < 0.05); and current density value of the primary cultured hippocampal neurons in serum of the RBE group and fluoxetine group decreased significantly (P < 0.01,P < 0.01).It was concluded that RBE can effectively correct the abnormal 5-HT3R channel currents of rats with depression emotion,which may be one of the central mechanisms in the treatment of depression emotion.
