Effects on Proliferation Inhibition, Apoptosis and Secretion of Extracellular Matrix Function of Human Hepatic Stellate Cell by Plumbagin
10.11842/wst.2015.11.015
- VernacularTitle:白花丹醌对人肝星状细胞的增殖抑制、凋亡及细胞外基质分泌功能的影响
- Author:
Yue PENG
;
Weina MIAO
;
Tiejian ZHAO
;
Guiyu LI
- Publication Type:Journal Article
- Keywords:
Plumbagin;
human hepatic stellate cell;
proliferation inhibition;
apoptosis;
extracellular matrix
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2015;(11):2272-2279
- CountryChina
- Language:Chinese
-
Abstract:
This article was aimed to study the effects of plumbagin to human hepatic stellate cells.Observations were made on the influence of proliferation inhibition rate,apoptosis,secretion of extracellular matrix function and matrix metalloproteinase (MMP) by plumbagin.HSC-LX2 and drug were co-incubated for 48 hours.Then,MTT assay was used in the detection of inhibition of cell proliferation.The flow cytometry was used in the detection of apoptosis.Immunohistochemical method was used to observe typeⅠ and Ⅲ collagen,MMP-1 and MMP-13 expression location and area.The results showed that the low,medium and high concentrations of plumbagin inhibited cell proliferation rate of HSC-LX2,induced apoptosis of cells,reduced the secretion of typeⅠ and Ⅲ collagen,and increased the secretion ability of MMP-1 and MMP-13.Effects mentioned above were dose-dependent with statistical difference (P < 0.05).Effects in the medium and high concentrations groups were stronger than colchicine group.It was concluded that plumbagin had the ability to inhibit cell proliferation rate of HSC-LX2,induce apoptosis,reduce the secretion of extracellular matrix,and increase the secretion ability of fibrin degradation enzyme.Therefore,it had intervention effect on the process of liver fibrosis.All effects mentioned above were dose-dependent.And effects in the medium and high concentrations groups were stronger than colchicine group.
