Effects of Different Preparation Methods on Content of Ginsenosides Rg1, Re and Rb1 in Yi-Xin-Shu Tablets by HPLC-ELSD
10.11842/wst.2015.11.016
- VernacularTitle:HPLC-ELSD方法研究不同样品处理方式对益心舒片中人参皂苷Rg1、Re和Rb1含量测定的影响
- Author:
Hongmei YIN
;
Dianhong XU
;
Juan FU
;
Fengjun HE
;
Junchao LIU
;
Yongcheng SUN
;
Wei XIAO
- Publication Type:Journal Article
- Keywords:
Yi-Xin-Shu tablets;
Panax ginseng;
ginsenosides;
HPLC-ELSD
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2015;(11):2280-2284
- CountryChina
- Language:Chinese
-
Abstract:
This paper was aimed to study effects of different preparation methods on the content of ginsenosides Rg1,Re and Rb1 in Yi-Xin-Shu (YXS) tablets by HPLC-ELSD.HPLC-ELSD was used as the detection method.The separation and content of ginsenosides Rg1,Re and Rb1 were used as indexes.The influences of three different preparation methods (i.e.,defatted alcohol extraction and butanol extraction,alcohol extraction and butanol extraction,alcohol extraction and butanol extraction ammonia solution washing) on the effect of YXS tablets were studied.Then,the same content determination method was used to compare the influence of alkali washing treatment to ginsenosides Rg1,Re and Rb1 among different batches of Panax ginseng.The results showed that a good separation of ginsenosides Rg1,Re and Rb1 component peak of YXS tablets was achieved by three kinds of separation methods.The separation degree was greater than 1.5.Ammonia solution washing had some effect on ginsenosides Rg1,Re and Rb1 content,which made the content of ginsenosides Rg1,Re and Rb1 be 1.5-1.8 times to those without alkali washing.No effect was shown on the content of ginsenosides Rg1,Re and Rb1 during ammonia solution washing.It was concluded that some other ginsenosides can be transferred into ginsenosides Rg1,Re and Rb1 in YXS tablets solution after ammonia solution washing.
