Optimization on Extraction Technology of Shu-Feng Ding-Chuan Granules in Huang-Qin Group by Box-Behnken Response Surface Methodology
10.11842/wst.2015.11.018
- VernacularTitle:Box-Behnken响应面法优化疏风定喘颗粒黄芩组的提取工艺研究
- Author:
Ruihua LI
;
Ying LI
;
Yongxiang WANG
;
Yun WU
;
Gang DING
- Publication Type:Journal Article
- Keywords:
Shu-Feng Ding-Chuan granules;
response surface methodology;
extraction technology;
single factor experiment;
baicalin;
praeruptorin A
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2015;(11):2290-2295
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to optimize the extraction technology of Shu-Feng Ding-Chuan(SFDC) granules in the Huang-Qin(HQ)group by Box-Behnken response surface methodology(RSM).With three major characteristic components (baicalin,praeruptorin A and solid content in extraction liquid),the effects of four factors,such as the concentration of ethanol,the dosage of ethanol,the duration of extraction and the extraction times,were investigated by the single factor experiment.Then,the range of parameters of key factors was further studied and explored by Box-Behnken RSM.The results of single factor experiment and Box-Behnken design showed that the optimum preparation conditionswere 8-fold 70% ethanol,extracted for 2 times,with 1.0 h per each time.Under these conditions,the transfer rates of baicalin and praeruptor in A were 89.57% and 87.90%,respectively.And the transfer rateof solid content was 32.35%.It was concluded that the single factor experiment combined with RSM can be used in theoptimization of extraction technology for SFDC granules in the HQ group.This technique wasstable and feasible,which provided scientific evidences for the industrial production.
