Study on Inspection Method for Limited Quantity of Aconitine and Content Determination Method of Schisandrol in Yi-Shen Xiao-Zhong Pill
10.11842/wst.2015.11.019
- VernacularTitle:益肾消肿丸中乌头碱限量检查方法和五味子醇甲含量测定研究
- Author:
Xiuling JIA
;
Muzi LI
;
Lin QIU
;
Ying LIU
- Publication Type:Journal Article
- Keywords:
Yi-Shen Xiao-Zhong pill;
aconitine;
schisandrol;
HPLC
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2015;(11):2296-2299
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to establish an inspection method for the limited quantity of aconitine and the content determination of schisandrol in Yi-Shen Xiao-Zhong(YSXZ) pill.Thin layer chromatography (TLC) was used to inspect the limited quantity of aconitine in YSXZ pill.The content of schisandrol was determined by high-performance liquid chromatography (HPLC).The results showed that the identification method of the limited quantity of aconitine and the content determination method of schisandrol were established.Compared to the correspondent position of the control chromatography,the limited quantity of aconitine was 2.5μg,which was much lower than the dose of inducing toxic reaction.It can be used in the limited quantity control of aconitine for toxic reaction in YSXZ pill.The calibration curve of schisandrol was linear at 2.92-95.36μg·mL-1.The regression equation wasy = 18.630x + 2.556 (r = 1).The average recovery was 95.10% and RSD was 1.55% (n = 6).It was concluded that the established method for the inspection and content determination of aconitine and schisandrol was simple,accurate and reproducible.It can be used in the quality control of YSXZ pill.
