Rapid Determination of Timosaponin B Ⅱ in Anemarrhenae Rhizomaby NIRS with HPLC
10.11842/wst.2015.11.021
- VernacularTitle:NIRS与HPLC结合快速测定知母中知母皂苷BⅡ的含量
- Author:
Huiying XIE
;
Bo SHI
;
Fengqing WANG
;
Jingwei LEI
;
Caixia XIE
;
Lina WANG
- Publication Type:Journal Article
- Keywords:
NIRS;
HPLC;
concentration determination;
Anemarrhenae Rhizoma;
timosaponin BⅡ
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2015;(11):2306-2311
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to establish a rapid detection method for timosaponin BⅡ in Anemarrhenae Rhizoma in order to determine its concentration quickly,conveniently and efficiently.The concentration of timosaponin BⅡ in A.Rhizomadetected by HPLC in the Chinese Pharmacopeia was used as the actual measured value.The near-infrared spectroscopy (NIRS) was used to collect the spectrogram of A.Rhizomasamples.The partial least squares (PLS) of TQ Analyst 8.0 were used in the data analysis.Through the pretreatment,wavelength range and principal component number selection,the actual measured value and NIRS information were associated for the establishment of the optimal quantitative analysis model of timosaponin BⅡ.The results showed that the correlation coefficients (R2),root-mean-square error of calibration (RMSEC),root-mean-square error of prediction (RMSEP),root-mean-square error of cross-validation (RMSECV) and the performance index (PI) of the established model were 0.975 15,0.094 2,0.080 0,0.369 20,and 91.0,respectively.It was concluded that the established quantitative analysis model by NIRS with HPLC was able to determine the concentration of timosaponin BⅡ in A.Rhizomaquickly and accurately.
