Research Method on Content Determination of Strychnine and Brucine in Rat Plasma
10.11842/wst.2015.11.027
- VernacularTitle:测定大鼠血浆中士的宁和马钱子碱含量的方法研究
- Author:
Tianchao CHEN
;
Li LI
;
Qichao LIANG
;
Wei CHENG
- Publication Type:Journal Article
- Keywords:
Strychnine;
brucine;
UPLC;
biopharmaceutical analysis
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2015;(11):2341-2348
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to establish an effective method for the simultaneous content determination of strychnine and brucine in rat plasma.The Wistar rat plasma samples were processed by liquid-liquid extraction and separated on a UPLC BEH C18 column.The mobile phase was acetonitrile - acid water (17?83).The acid water was 0.02 mol·L-1.The potassium dihydrogen phosphate and 0.014 8 mol·L-1 sodium heptane sulfonate was mixed with equal amount.The 10% phosphonic acid was used to adjust the system to the pH of 2.8.The detection wavelength was set at 260 nm.The results showed that foreign materials in the Wistar rat plasma did not interfere with the content determination of samples.The calibration curve of strychnine was in good linearity within the range of 0.067 84-3.392 00μg·mL-1.The calibration curve of brucine was in good linearity with the range of 0.052 48-2.624 00μg·mL-1.The intra-and inter-day precisions were less than 10%.The mean extraction recoveries were more than 85%.The stability results met the requirements for biopharmaceutical analysis.It was concluded that the UPLC detection method was sensitive and precise,which can be used in the pharmacokinetics study of strychnine and brucine in Tong-Mai(TM) pill.
