The Effect of Scutellaria Baicalensis Water Extract on Proliferation, Cytokines mRNA Expressions and Promoter Activity of 3T3-L1 Cells
10.11842/wst.2015.11.030
- VernacularTitle:黄芩水提液对3T3-L1脂肪细胞增殖、诱导分化及脂联素启动子荧光素酶活性的影响
- Author:
Lin CUI
;
Lingling LU
;
Qiang LI
;
Junhua ZAI
;
Weihong LIU
;
Xiaoxiao WANG
- Publication Type:Journal Article
- Keywords:
Scutellaria baicalensis;
3T3-L1 cells;
real-time PCR;
cytokines;
luciferase activity
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2015;(11):2360-2366
- CountryChina
- Language:Chinese
-
Abstract:
The study was designed to measure the effect of S.baicalensiswater extract (SBWE) on 3T3-L1 cells and its adiponectin (ADP) mRNA (Adipoq) and promoter luciferase activity.Cell survival rate was determined by MTT assay.The expression of Adipoq was measured by real-time PCR,while the luciferase report systems of Adipoq were used to transfer 3T3-L1 cells.The luciferase activities of the transferred cells were compared by luciferase assay.It was found that the mRNA expression of Adipoq was decreased in comparison with the control group.The luciferase activity showed a stronger ADP promoter activity in 3T3-L1 cells in SBWE treated group than that of control one.In conclusion,SBWE treatment improves the cykomine expression and luciferase reporter gene activity which will be an essential method for further studies of obesity therapy in traditional Chinese medicine.
