Effect of AngⅡ on Insulin Gene Expression in RIN-m Cells and Its Molecular Mechanism
- VernacularTitle:血管紧张素Ⅱ对RIN-m细胞胰岛素基因表达的影响及其分子机制研究
- Author:
Lu XIN
;
Zhang HUA
- Publication Type:Journal Article
- Keywords:
AngⅡ;
RIN-m cell;
Insulin gene expression;
Molecular mechanism
- From:
China Pharmacist
2015;18(12):2025-2029
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To discuss the effect of AngⅡ on insulin gene expression IN RIN-m cells and its molecular mechanism. Methods:RIN-m cells were cultured and divided into three groups, including the control group, 100 nmol·L-1 AngⅡ group and losartan pretreatment group. After 24-hour incubation, insulin gene expression in RIN-m cells was detected by RT-PCR, the mean flu-orescent intensity of 2', 7'-dichlorofluorescein ( DCF) was detected by flow cytometry, PDX-1 and MafA mRNA expression were detec-ted by RT-PCR and the protein expression was detected by Western-blot. Results: Insulin expression in RIN-m cells, cellular ROS level, PDX-1 expression and MafA expression in 100 nmol · L-1 AngⅡ group were significantly different from those in the control group and losartan pretreatment group (P<0. 05). The later two groups had no significant differentces in those indices (P>0. 05). Conclusion:AngⅡ can down-regulate PDX-1 and MafA expression inβ-cells through oxidative stress pathway, and then inhibit insu-lin gene expression. Pretreatment with losartan can antagonize the effect of AngⅡ, and has protective effect onβ-cells in the aspect of insulin gene expression.
