Study on the HPLC Fingerprint of Glycyrrhizae Dispensing Granules
- VernacularTitle:甘草配方颗粒HPLC指纹图谱研究
- Author:
Jiangping ZHENG
;
Jun LIANG
;
Liangyong HUANG
- Publication Type:Journal Article
- Keywords:
Glycyrrhizae;
Dispensing granules;
HPLC;
Fingerprint;
Liquiritin;
Glycyrrhizic acid
- From:
China Pharmacist
2015;18(12):2053-2057
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish an HPLC method for the fingerprint determination of glycyrrhizae dispensing granules. Meth-ods:Twelve samples were analyzed by HPLC with glycyrrhizic acid as the reference. The analysis was performed on a Waters SunFire C18 column(250 mm × 4. 6 mm,5 μm) with the mobile phase A of acetonitrile and the mobile phase B of 0. 1% phosphoric acid solu-tion with gradient elution. The flow rate was 1. 0 ml·min-1 , the detection wavelength was 237nm, and the column temperature was 30℃. Results:By analyzing the fingerprint, 21 peaks existed including the peak of liquiritin and glycyrrhizic acid. The similarity of the samples was more than 0. 97. Conclusion:The established HPLC fingerprint of glycyrrhizae dispensing granules is dependable and simple. The method can provide scientific basis for the quality control of glycyrrhizae dispensing granules.
