Role of miR-17 in human glioma U87 cells invasion by regulating MMP-2 expression
10.7644/j.issn.1674-9960.2015.11.003
- VernacularTitle:miR-17靶向调控 MMP-2基因对人脑胶质瘤 U87细胞侵袭性的影响
- Author:
Qingqing ZHOU
;
Zhenxing ZHANG
;
Chenguang LI
;
Yang LI
;
Honglei WANG
- Publication Type:Journal Article
- Keywords:
microRNA;
MMP-2;
human glioma U87 cells
- From:
Military Medical Sciences
2015;(11):821-824
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify the role of microRNA-17(miR-17)in human glioma U87 cells invasion which may regulate expression of matriv metalloproteinase(MMP)-2.Methods U87 cells were cultured in vitro,while changes in cellular morphology were observed by phase contrast microscope.The miR-17 which might regulate the expression of MMP-2 was predicted by bioinformatics and identified using dual luciferase report system.Expressions of miR-17 and MMP-2 were determined using real-time PCR and Western blot after transfection of miR-17 mimics.The invasion of U87 cells was detected in vitro by Transwell chamber.Results Expression of MMP-2 was positive by immunofluorescence cytochemistry. Using dual luciferase reporter system,miR-17 could inhibit the expression of MMP-2 by binding to its mRNA 3′UTR. Results of real-time PCR and Western blot showed that over-expression of miR-17 down-regulated expression of MMP-2. The invasion of U87 cells was suppressed by over-expression of miR-17.Conclusion MiR-17 may negatively regulate expression of MMP-2 in human glioma U87 cells and inhibit cell invasion.
