A study on enrichment and purification of core fucose glycosylation and its immunity properties in patients with schizophrenia
10.3969/j.issn.1673-4130.2015.23.029
- VernacularTitle:精神分裂症患者免疫球蛋白核心岩藻糖基化组分的富集纯化及免疫学性质分析
- Author:
Jing GUO
;
Jinyun QIU
;
Fangbo FENG
- Publication Type:Journal Article
- Keywords:
schizophrenia;
immunoglobulins;
core fucosylation;
immunoglobulin G subclass
- From:
International Journal of Laboratory Medicine
2015;(23):3433-3434
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a platform for enrichment and purification of core fucose glycosylation and analyse its immu‐nity properties .Methods Serum immunoglobulin G(IgG) was seperated and purified by using protein G cross‐linked sepharose ,and core fucose glycosylation components of IgG were enriched and purified by using lens culinaris agglutinin (LCA)affinity chromatog‐raphy ,meanwhile ,the isoforms of LCA‐IgG were determined by using Western blotting .Results The purification rate of serum IgG was 62% and yield rate of LCA‐IgG was 4 .59% .Serum IgG1 ,IgG2 and IgG3 were identified except IgG4 in patients with schizo‐phrenia ,while serum IgG1 ,IgG2 ,IgG3 and IgG4 were all identified in healthy individuals .Conclusion A method for enrichment and purification of core fucose glycosylation in patients with schizophrenia have been successfully established and distribution character‐istics of the LCA‐IgG isoforms have been determined ,which could provide a new insight into immune pathomechanism of schizo‐phrenia .
